| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | JENSEN, Kali | FR | Wright City High School | NT | Unverified: |
| 3 | STOCKMANN, Gretchen | FR | Wright City High School | NT | Unverified: |
| 4 | DACE, Sophia | FR | Sullivan High School | 33.11 | Verified |
| 5 | APEL, Kaydence | JR | Helias Catholic High School | 34.11 | Verified |
| 6 | WEBER, Mattalyn | FR | St. Francis Borgia High School | NT | Unverified: |
| 7 | FLEER, BAILEY | SO | New Haven High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DUNN, Peyton | SO | St. Clair High School | 32.30 | Verified |
| 2 | GUEHNE, MORGAN | SO | New Haven High School | 31.05 | Verified |
| 3 | GRONER, Isabella | SO | New Haven High School | 30.37 | Verified |
| 4 | STEELE, Gracie | JR | New Haven High School | 29.74 | Verified |
| 5 | STRUBBERG, Avery | SO | New Haven High School | 29.97 | Verified |
| 6 | MCCLAIN, Daveah | SO | Sullivan High School | 30.42 | Verified |
| 7 | FRENCH, Marlee | FR | Helias Catholic High School | 31.94 | Verified |
| 8 | CARPENTER, Makenzie | JR | Sullivan High School | 32.49 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MURRAY, Alexis | JR | St. Clair High School | 29.31 | Verified |
| 2 | SCHATZLER, Cylee | SO | St. Clair High School | 28.92 | Verified |
| 3 | HARRISON, Kenadi | SO | Helias Catholic High School | 28.23 | Verified |
| 4 | STRUEMPH, Isabelle | JR | Helias Catholic High School | 26.20 | Verified |
| 5 | RILEY, Teagen | JR | Wright City High School | 28.09 | Verified |
| 6 | HEADY, Eva | JR | Sullivan High School | 28.30 | Verified |
| 7 | SMITH, Marina | JR | Sullivan High School | 28.97 | Verified |
| 8 | MOELLERS, Lydia | SO | Helias Catholic High School | 29.71 | Verified |