| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. Francis Borgia High School | A | Dowil | Rondot | Jacobsen | Smith | NT | Unverified: |
| 3 | Wright City High School | A | NT | Unverified: | ||||
| 4 | Helias Catholic High School | B | Vallon | Mason | Jahnsen | Lock | 52.64 | Verified |
| 5 | Helias Catholic High School | A | Struemph | Dollar | French | Howell | 52.64 | Verified |
| 6 | Sullivan High School | A | Carpenter | Dace | Garner | Peterson | NT | Unverified: |
| 7 | Wright City High School | B | NT | Unverified: |