| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Christian Fellowship School | A | Horn | Kauffman | McGowan | Freeman | 3:58.36 | Verified |
| 2 | Hickman High School | A | Backues | Current | Fletcher | Yoon | 3:45.21 | Unverified: |
| 3 | Rock Bridge High School | A | Chinikoski | Hockman | Scott | Bailey | 3:40.00 | Unverified: |
| 4 | Capital City High School | A | Breuer | Bonchonsky | Johnson | Barnes | 3:29.73 | Verified |
| 5 | Hickman High School | C | Johnson | Lee | Bullock | Renoe | 3:38.21 | Unverified: |
| 6 | Hickman High School | B | Brinkman | Muhlenbruck | Villasana | Waller | 3:40.21 | Unverified: |
| 7 | Battle High School | A | Alderton | Gordon | Dennison | Rice | 3:50.00 | Unverified: |
| 8 | Lighthouse Preparatory Academy | A | Keeney | Brooks | Hoselton | SCHRIMPF | DNS | Unverified: |