| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Lighthouse Preparatory Academy | A | Hutchison | Thompson | Bethards | Brooks | 5:50.17 | Verified |
| 3 | Hickman High School | A | Bruce | Eastman | McNally | Parker | 4:30.52 | Unverified: |
| 4 | Capital City High School | A | Lutz-Jenkins | Cofer | Ndegwa | Mays | 4:23.91 | Verified |
| 5 | Battle High School | A | Johnson | Tydings | May | Glay | 4:30.00 | Unverified: |
| 6 | Hickman High School | B | Bukoski | Keenoy | O'Laughlin | Weber | DNS | Unverified: |