| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | STEMMONS, Sy'Rae | SO | Hickman High School | 2:33.12 | Unverified: |
| 2 | LEIGH, Sophia | JR | Hickman High School | 2:35.57 | Verified |
| 3 | O'LAUGHLIN, Zoe | FR | Hickman High School | 2:38.93 | Verified |
| 4 | KEENOY, Natalie | FR | Hickman High School | 2:41.55 | Verified |
| 5 | MCNALLY, Nora | SO | Hickman High School | 2:46.82 | Verified |
| 6 | DOMEIER, Isabelle | SO | Hickman High School | 2:48.35 | Verified |
| 7 | HANER, Avery | JR | Battle High School | 2:49.45 | Verified |
| 8 | IRELAND, Isabelle | FR | Rock Bridge High School | 2:49.60 | Verified |
| 9 | PARKER, Emily | JR | Hickman High School | 2:55.34 | Verified |
| 10 | WEBER, Iris | FR | Hickman High School | 2:55.92 | Verified |
| 11 | SCHULTE, Ellie | FR | Capital City High School | 3:02.30 | Verified |
| 12 | COLEMAN, Jayden | SO | Battle High School | 3:03.41 | Verified |
| 13 | CASTRO, Ezra | FR | Battle High School | 3:09.24 | Verified |
| 14 | CHEN, Ria | SR | Capital City High School | 3:09.54 | Verified |
| 15 | BERNSKOETTER, Addison | FR | Capital City High School | 3:19.07 | Verified |
| 16 | BACON, Makenzie | SO | Lighthouse Preparatory Academy | 3:34.53 | Verified |
| 17 | BRUCE, Addyson | FR | Hickman High School | NT | Unverified: |
| 18 | BUKOSKI, Emma | FR | Hickman High School | NT | Unverified: |
| 19 | BRAGG, Abigail | FR | Christian Fellowship School | NT | Unverified: |