| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | BAKER, Lucy | SO | Christian Fellowship School | NT | Unverified: |
| 3 | DICKNEITE, Matty | FR | Lighthouse Preparatory Academy | NT | Unverified: |
| 4 | LYSON, Mikayla | FR | Rock Bridge High School | 54.68 | Verified |
| 5 | WARREN, Leah | JR | Capital City High School | 1:00.36 | Verified |
| 6 | KASTER, Lillian | SO | Hickman High School | NT | Unverified: |
| 7 | HUTCHISON, Madeline | SO | Lighthouse Preparatory Academy | NT | Unverified: |