| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | REDMOND-LILLY, O'Lissa | SO | Hickman High School | NT | Unverified: |
| 2 | BROOKS, Lizzy | SO | Lighthouse Preparatory Academy | 1:23.77 | Verified |
| 3 | BRASE, Kalley | SO | Capital City High School | 1:15.90 | Verified |
| 4 | NDEGWA, Elizabeth | JR | Capital City High School | 1:13.50 | Verified |
| 5 | KFURI, Julia | FR | Rock Bridge High School | 1:15.42 | Verified |
| 6 | SALL, Elizabeth | FR | Hickman High School | 1:18.00 | Verified |
| 7 | THOMPSON, Caitlyn | SR | Lighthouse Preparatory Academy | 1:27.05 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ZAPIEN, Aimarie | FR | Hickman High School | 1:09.67 | Verified |
| 2 | KEMNA, Kari | SO | Capital City High School | 1:09.28 | Verified |
| 3 | EASTMAN, Ellie | SO | Hickman High School | 1:05.32 | Verified |
| 4 | PETERSON, Athena | SO | Hickman High School | 1:00.94 | Verified |
| 5 | MALAND, Claire | FR | Hickman High School | 1:02.73 | Verified |
| 6 | IRELAND, Isabelle | FR | Rock Bridge High School | 1:08.90 | Verified |
| 7 | LUTZ-JENKINS, Kennedie | FR | Capital City High School | 1:09.46 | Verified |
| 8 | SCHULTZ, Mylisa | SO | Battle High School | 1:10.53 | Verified |