| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Battle High School | A | Christopher | Gordon | Weir | Robinson | 48.00 | Unverified: |
| 3 | Battle High School | B | Nelson | Cross | Rice | Harris | 47.00 | Unverified: |
| 4 | Rock Bridge High School | A | Atkinson | Hood | TRUITT | Grotewiel | DQ | Unverified: |
| 5 | Hickman High School | A | Hester | Johnson | McFarland | Thomas | 46.40 | Unverified: |
| 6 | Capital City High School | A | Bonchonsky | Austin | Barnes | Otten | 47.70 | Unverified: |