| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Rock Bridge High School | A | Lyson | Szydlowski | Shockley | Fisher | 53.15 | Unverified: |
| 4 | Capital City High School | A | Bibbs | Mays | Kuebler | Defeo | 52.11 | Verified |
| 5 | Battle High School | A | May | Johnson | Tydings | Carter | 53.00 | Unverified: |
| 6 | Battle High School | B | Patrick | Belcher-Avant | Perkey | George | 54.00 | Unverified: |