| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | JOHNSTON, Ellie | JR | Centralia (Christ Our Rock Lutheran) | 17.61 | |
| 4 | JOURDAN, Olivia | FR | Breese (Mater Dei) | 15.04 | |
| 5 | WEDEKEMPER, Lily | FR | Centralia (Christ Our Rock Lutheran) | 15.23 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | NISSEN, Zoe | SO | Freeburg | 14.35 | |
| 2 | FINLEY, Lilly | SO | O'Fallon (First Baptist Academy) | 14.02 | |
| 3 | ROWDEN, Kharyss | SO | Freeburg | 13.84 | |
| 4 | DISTLER, Molly | SO | Belleville (Althoff Catholic) | 13.43 | |
| 5 | SHERFY, Claire | JR | Edwardsville (Metro-East Lutheran) | 13.80 | |
| 6 | OHM, Charlotte | SO | Edwardsville (Metro-East Lutheran) | 13.92 | |
| 7 | RICKMAN, Ja'Niyah | SO | Belleville (Althoff Catholic) | 14.25 | |
| 8 | KAMPWERTH, Tori | SO | Breese (Mater Dei) | 14.44 |