| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Edwardsville (Metro-East Lutheran) | A | Daly | Stewart | Pryor | Goeckner | NT | |
| 3 | Freeburg | A | Merz | Jarvis | Thompson | Murphy | 1:43.25 | |
| 4 | Belleville (Althoff Catholic) | A | Nave | Lewis | Done | Clemons | 1:41.39 | |
| 5 | Breese (Mater Dei) | A | Hagen | Grapperhaus | Diekemper | Woomer | 1:42.00 | |
| 6 | Centralia (Christ Our Rock Lutheran) | A | Lu | Lyons | Morris | Lyons | NT |