| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Freeburg | A | Hurst | Stroder | Range | Dircks | 2:03.00 | |
| 4 | Breese (Mater Dei) | A | Becker | Jourdan | Zurliene | Faus | 2:00.00 | |
| 5 | Belleville (Althoff Catholic) | A | Thorpe | Rickman | Distler | Distler | 2:02.00 | |
| 6 | O'Fallon (First Baptist Academy) | A | Hagadorn | Finley | Nava | Nava | NT |