| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Edwardsville (Metro-East Lutheran) | A | Fields | Holmes | Pryor | Schlessinger | 3:56.00 | |
| 4 | Freeburg | A | Duffie | Pluff | Banks | Frech | 3:34.22 | |
| 5 | Belleville (Althoff Catholic) | A | Schallert | Tell | Schallert | Cole | 3:55.00 |