| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | MORRIS, Alijah | FR | Centralia (Christ Our Rock Lutheran) | NT | |
| 3 | LYONS, Liagel | FR | Centralia (Christ Our Rock Lutheran) | 31.34 | |
| 4 | HILMES, Luke | SO | Breese (Mater Dei) | 27.64 | |
| 5 | JACKSON, Marquion | JR | Belleville (Althoff Catholic) | 27.66 | |
| 6 | KOHLMILLER, Griffin | JR | Edwardsville (Metro-East Lutheran) | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CHERNIANSKII, Mark | JR | Edwardsville (Metro-East Lutheran) | 25.95 | |
| 2 | COOPER, Michael | JR | Freeburg | 25.54 | |
| 3 | TEETS, Landon | SO | Breese (Mater Dei) | 24.80 | |
| 4 | MURPHY, Carter | SO | Breese (Mater Dei) | 23.90 | |
| 5 | GORING, Daniel | JR | Breese (Mater Dei) | 24.10 | |
| 6 | COLDON, Charleston | SO | Belleville (Althoff Catholic) | 25.00 | |
| 7 | ZHU, Edison | JR | Freeburg | 25.90 | |
| 8 | LIGHT, Bryce | FR | O'Fallon (First Baptist Academy) | 26.84 |