| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | CUNNINGHAM, Tyrani Unique | SO | Belleville (Althoff Catholic) | 37.29 | |
| 4 | JOHNSTON, Ellie | JR | Centralia (Christ Our Rock Lutheran) | 35.18 | |
| 5 | HAGADORN, Lydia | SO | O'Fallon (First Baptist Academy) | 35.55 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HEBEL, Kennadi | SR | Belleville (Althoff Catholic) | 32.00 | |
| 2 | HUGHES, Emily | JR | Edwardsville (Metro-East Lutheran) | 31.63 | |
| 3 | HURST, Samantha | SO | Freeburg | 30.00 | |
| 4 | SCHULZ, Greta | SR | Freeburg | 29.00 | |
| 5 | OHM, Charlotte | SO | Edwardsville (Metro-East Lutheran) | 29.78 | |
| 6 | KAMPWERTH, Tori | SO | Breese (Mater Dei) | 30.04 | |
| 7 | FAUS, Elisa | SO | Breese (Mater Dei) | 31.94 | |
| 8 | WILLIAMS, Catherine | SO | O'Fallon (First Baptist Academy) | 32.74 |