| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KAMPWERTH, Alexis | SR | Breese (Mater Dei) | 5:40.59 | |
| 2 | MCKOWEN, Sarah | JR | Centralia (Christ Our Rock Lutheran) | 5:51.18 | |
| 3 | SCHEIBEL, Kiersten | SR | Belleville (Althoff Catholic) | 6:09.71 | |
| 4 | BRAMMEIER, Rylee | SO | Centralia (Christ Our Rock Lutheran) | 6:13.09 | |
| 5 | HACKER, Isabella | SO | Breese (Mater Dei) | 6:15.00 | |
| 6 | COX, Elisabeth | SR | Freeburg | 6:26.59 | |
| 7 | BARCELO MACHADO, Mireia | SO | Edwardsville (Metro-East Lutheran) | 6:48.87 | |
| 8 | LIN, Ashley | FR | Freeburg | 6:58.50 |