| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Freeburg | A | Rozhon | Cooper | Duffie | LaChance | 11:35.00 | |
| 2 | Belleville (Althoff Catholic) | A | Schillinger | Cole | Clemons | Talafuse | 12:15.00 | |
| 3 | Centralia (Christ Our Rock Lutheran) | A | Lyons | Koch | Lu | Gregory | 12:16.00 | |
| 4 | Breese (Mater Dei) | A | Holthaus | Huelsmann | Shelton | Goebel | NT | |
| 5 | Edwardsville (Metro-East Lutheran) | A | Broekemeier | Goeckner | Staake | Weber | NT | |
| 6 | O'Fallon (First Baptist Academy) | A | Clydesdale | Light | Miller | Lipe | NT |