| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | PALMA, Julio | SR | Breese (Mater Dei) | NT | |
| 4 | HAAR, Ryan | JR | Belleville (Althoff Catholic) | 1:09.33 | |
| 5 | MORRIS, Alijah | FR | Centralia (Christ Our Rock Lutheran) | NT | |
| 6 | PALMA, Andrew | SO | Breese (Mater Dei) | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LU, Yilin | SR | Centralia (Christ Our Rock Lutheran) | NT | |
| 2 | FIELDS, Josh | JR | Edwardsville (Metro-East Lutheran) | 57.17 | |
| 3 | MEYER, Logan | SR | Freeburg | 56.00 | |
| 4 | CLEMONS, Ravonne | SR | Belleville (Althoff Catholic) | 53.46 | |
| 5 | DUFFIE, Zach | FR | Freeburg | 54.00 | |
| 6 | PRYOR, Carter | JR | Edwardsville (Metro-East Lutheran) | 56.85 | |
| 7 | LIGHT, Bryce | FR | O'Fallon (First Baptist Academy) | 1:03.74 | |
| 8 | MILLER, Caleb | SO | O'Fallon (First Baptist Academy) | 1:08.39 |