| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | ZAGHINI, Shaliny | SR | Belleville (Althoff Catholic) | 1:20.00 | |
| 4 | WILLIAMS, Catherine | SO | O'Fallon (First Baptist Academy) | 1:13.53 | |
| 5 | WEDEKEMPER, Lily | FR | Centralia (Christ Our Rock Lutheran) | 1:15.00 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BECKER, Ryann | JR | Breese (Mater Dei) | 1:10.86 | |
| 2 | NELSON, Eris | SR | Belleville (Althoff Catholic) | 1:10.00 | |
| 3 | FAUS, Elisa | SO | Breese (Mater Dei) | 1:09.07 | |
| 4 | RANGE, Anna | SO | Freeburg | 1:09.00 | |
| 5 | STRUBHART, Reese | JR | Breese (Mater Dei) | 1:09.00 | |
| 6 | ROWDEN, Kharyss | SO | Freeburg | 1:09.53 | |
| 7 | NAVA, Isabela | SO | O'Fallon (First Baptist Academy) | 1:10.21 |