| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Breese (Mater Dei) | A | Jourdan | Rakers | Diekemper | Goring | 1:37.00 | |
| 4 | Belleville (Althoff Catholic) | A | Cole | Nave | Done | Lewis | 1:34.68 | |
| 5 | Freeburg | A | Thompson | Banks | Frech | Pluff | 1:35.40 | |
| 6 | Edwardsville (Metro-East Lutheran) | A | Schlessinger | Chernianskii | Holmes | Kohlmiller | 1:38.12 |