| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | O'Fallon (First Baptist Academy) | A | Nava | Hagadorn | Finley | Nava | 2:06.40 | |
| 4 | Belleville (Althoff Catholic) | A | Thorpe | Reyes-Cruz | Distler | Distler | 1:57.69 | |
| 5 | Freeburg | A | Trentman | Geluck | Trentman | Harmon | 1:58.82 |