| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MCKOWEN, Sarah | JR | Centralia (Christ Our Rock Lutheran) | 2:32.22 | |
| 2 | EMIG, Reid | FR | Breese (Mater Dei) | 2:46.03 | |
| 3 | SCHNEIDER, Emma | JR | Freeburg | 3:05.44 | |
| 4 | BARCELO MACHADO, Mireia | SO | Edwardsville (Metro-East Lutheran) | 3:05.55 | |
| 5 | NAVA, Gabriela | SO | O'Fallon (First Baptist Academy) | 3:13.50 | |
| 6 | HAFNER, Hannah | JR | Belleville (Althoff Catholic) | 3:17.22 | |
| 7 | SPANLEY, Jenna | JR | Freeburg | 3:21.82 | |
| 8 | WILLIAMS, Catherine | SO | O'Fallon (First Baptist Academy) | NT | |
| 9 | NAPOVANICE, Reagan | FR | Breese (Mater Dei) | NT |