| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 7 | Belleville (Althoff Catholic) | A | Scheibel | Hafner | Zaghini | Nelson | 14:45.00 | |
| 8 | Freeburg | A | Drueke | Dircks | Lin | Wesselmann | 17:10.00 | |
| 9 | Breese (Mater Dei) | A | Hacker | Kampwerth | Emig | Kampwerth | NT | |
| 10 | Centralia (Christ Our Rock Lutheran) | A | Nolte | Wedekemper | Brammeier | McKowen | NT |