| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | RAMMACHER, Lilly | SR | Villa Duchesne High School | 1:07.36 | Verified |
| 3 | GILJUM, Kaylie | JR | St. Joseph's Academy | 1:02.86 | Verified |
| 4 | REYES-CRUZ, Cristina | FR | Belleville (Althoff Catholic) | 56.68 | Verified |
| 5 | MILLS, Margot | JR | Father Tolton Regional Catholic | 52.64 | Verified |
| 6 | SCHEINER, Allison | SO | Visitation Academy | 1:03.67 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BOOCK, Sarah | JR | Cor Jesu Academy | 54.25 | Verified |
| 2 | RICHART, Claire | SR | Visitation Academy | 53.88 | Verified |
| 3 | SIEBERT, Lydia | SO | Father Tolton Regional Catholic | 59.00 | Verified |
| 4 | ROLWES, Bridget | SR | Cor Jesu Academy | 50.17 | Verified |
| 5 | HANNEKEN, Madi | SR | St. Joseph's Academy | 50.41 | Verified |
| 6 | STANN, Kailyn | FR | Nerinx Hall High School | 53.34 | Verified |
| 7 | JACKNEWITZ, Analise | JR | Nerinx Hall High School | 54.01 | Verified |
| 8 | ELSTON, Brooke | SR | Rosati-Kain High School | 54.48 | Verified |