| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 2 | GRAY, Ethan | Youth Track and Field of Memph | NT |
| 3 | HAMILTON, Tayvion | NEA GOLD RUSH | NT |
| 4 | BENTLEY, Isaiah | Elite Feet Performance | NT |
| 5 | ROBINSON, Marcus | Southern Speed Youth Track And | NT |
| 6 | SLATER, Martez | EDA FLYERS YOUTH TRACK TEAM | NT |
| 7 | HIGGINS, Teshawn | Elite Feet Performance | NT |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | BROOKS, Edward | Elite Feet Performance | 57.00 |
| 2 | TULLISON, Cedric | Fast & Furious | 56.00 |
| 3 | FOX, William | Revelation Track Club | 55.77 |
| 4 | BOYKIN, Jaylen | Revelation Track Club | 55.21 |
| 5 | ALEXANDER, Jaylen | NEA GOLD RUSH | 55.21 |
| 6 | BRATCHER, Corry | Elite Feet Performance | 56.00 |
| 7 | HENDRIX, Kielyn | NEA GOLD RUSH | 56.00 |
| 8 | SCHERROD JR, Devin | Elite Feet Performance | 59.00 |