| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | Lauren SPENCER | KANSAS CITY ELITE COMPETITIO | NT | |
| 3 | E'Myne ANDERSON | KANSAS CITY ELITE COMPETITIO | NT | |
| 4 | Avery DAWSON | WHATEVER IT TAKES | 58.91 | |
| 5 | Faimon ABIGAIL | KC NOVAS | 1:03.00 | |
| 6 | Raven BUCKLES | KANSAS CITY ELITE COMPETITIO | NT | |
| 7 | Chondra WALTERS | KANSAS CITY ELITE COMPETITIO | NT |