| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | Chondra WALTERS | KANSAS CITY ELITE COMPETITIO | NT | |
| 3 | Dasia PARRISH | PIRATE TRACK CLUB | NT | |
| 4 | E'Myne ANDERSON | KANSAS CITY ELITE COMPETITIO | NT | |
| 5 | Raven BUCKLES | KANSAS CITY ELITE COMPETITIO | NT | |
| 6 | Damiya RICHARDS | WHATEVER IT TAKES | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | Liv GUTIERREZ | WHATEVER IT TAKES | 27.80 | |
| 3 | Suzanna DANSEL | WHATEVER IT TAKES | 27.01 | |
| 4 | Avery DAWSON | WHATEVER IT TAKES | 25.86 | |
| 5 | Faimon ABIGAIL | KC NOVAS | 26.80 | |
| 6 | Jasmine PIPPINS | KC NOVAS | 27.67 |