| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | Braelyn SENA | GE LIGHTNING TRACK AND FIELD | NT | |
| 3 | Aurretta LYTLE | PIRATE TRACK CLUB | NT | |
| 4 | Jada ECHOLS | WHATEVER IT TAKES | 12.50 | |
| 5 | Grace ERB | KC NOVAS | NT | |
| 6 | Kennedi RANE | KANSAS CITY ELITE COMPETITIO | NT | |
| 7 | Donnise YOUNG | KANSAS CITY ELITE COMPETITIO | NT |