| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | Zoey REEVES | KC NOVAS | NT | |
| 2 | Raquel MABRY | WHATEVER IT TAKES | NT | |
| 3 | Isabella DEJOHN | GE LIGHTNING TRACK AND FIELD | NT | |
| 4 | Gabrielle BASS | PIRATE TRACK CLUB | 15.82 | |
| 5 | Lylah JOHNSTON | GE LIGHTNING TRACK AND FIELD | 16.00 | |
| 6 | Kaedyn LEWIS | KANSAS CITY ELITE COMPETITIO | NT | |
| 7 | Aaliyah MITCHELL | KANSAS CITY ELITE COMPETITIO | NT | |
| 8 | Maya SHENOY | KC NOVAS | NT |