| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | Gracie JACOBS | ST. TERESA'S ACADEMY | 5:14.44 | Verified |
| 2 | Amelia ARRIETA | ST. TERESA'S ACADEMY | 5:14.73 | Verified |
| 3 | Allie GRIFFITHS | WARRENSBURG HIGH SCHOOL | 5:54.27 | Verified |
| 4 | Peyton BRUNER | WARRENSBURG HIGH SCHOOL | 5:55.23 | Verified |
| 5 | Allie RAINS | NEVADA HIGH SCHOOL | 5:59.95 | Verified |
| 6 | Ciaira MARTINEZ | GRANDVIEW HIGH SCHOOL | 6:22.05 | Verified |
| 7 | Mya DANIELS | GRANDVIEW HIGH SCHOOL | 6:30.95 | Verified |
| 8 | Ellie BECK | PLEASANT HILL HIGH SCHOOL | 6:33.46 | Verified |
| 9 | Mary-Kate RUSSELL | NEVADA HIGH SCHOOL | 6:35.89 | Verified |
| 10 | Jaedyn BORJA | MARSHALL HIGH SCHOOL | 6:37.09 | Verified |
| 11 | Kiley FAUST | PLEASANT HILL HIGH SCHOOL | 6:41.78 | Verified |
| 12 | Elizabeth PAPRECK | MARSHALL HIGH SCHOOL | 6:46.10 | Verified |