| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | MARSHALL HIGH SCHOOL | A | DNS | Verified |
| 2 | NEVADA HIGH SCHOOL | A | DNS | Verified |
| 3 | GRANDVIEW HIGH SCHOOL | A | 3:38.22 | Verified |
| 4 | RUSKIN HIGH SCHOOL | A | 3:32.89 | Verified |
| 5 | WARRENSBURG HIGH SCHOOL | A | 3:33.67 | Verified |
| 6 | HARRISONVILLE HIGH SCHOOL | A | 3:40.60 | Verified |
| 7 | PLEASANT HILL HIGH SCHOOL | A | 3:48.31 | Verified |