| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | PLEASANT HILL HIGH SCHOOL | A | 1:35.71 | Verified |
| 2 | WARRENSBURG HIGH SCHOOL | A | 1:32.14 | Verified |
| 3 | CENTER HIGH SCHOOL | A | 1:31.12 | Verified |
| 4 | RUSKIN HIGH SCHOOL | A | 1:30.38 | Verified |
| 5 | HARRISONVILLE HIGH SCHOOL | A | 1:30.53 | Verified |
| 6 | GRANDVIEW HIGH SCHOOL | A | 1:31.12 | Verified |
| 7 | RAYTOWN SOUTH HIGH SCHOOL | A | 1:32.78 | Verified |
| 8 | NEVADA HIGH SCHOOL | A | 1:35.90 | Verified |