| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | PLEASANT HILL HIGH SCHOOL | A | 45.94 | Verified |
| 2 | RAYTOWN SOUTH HIGH SCHOOL | A | 44.58 | Verified |
| 3 | RUSKIN HIGH SCHOOL | A | 44.11 | Verified |
| 4 | GRANDVIEW HIGH SCHOOL | A | 42.83 | Verified |
| 5 | HARRISONVILLE HIGH SCHOOL | A | 42.96 | Verified |
| 6 | CENTER HIGH SCHOOL | A | 44.34 | Verified |
| 7 | WARRENSBURG HIGH SCHOOL | A | DNF | Verified |
| 8 | NEVADA HIGH SCHOOL | A | 46.04 | Verified |