| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | Natalia RYCHNOVSKY | SAINT MICHAEL CYOKS | 1:20.37 | |
| 2 | Kaydence HURLBERT | SAINT AGNES CYOKS | 1:18.85 | |
| 3 | Reagan MILES | SACRED HEART CYOKS | 1:15.85 | |
| 4 | Kenley DAVIS | HOLY TRINITY CYOKS | 1:13.01 | |
| 5 | Finlee FONSECA | SAINT JOSEPH CYOKS | 1:13.93 | |
| 6 | Colbie PATTISON | SACRED HEART CYOKS | 1:17.09 | changed from 1600M to 400M |
| 7 | Kelly NEENAN | SAINT AGNES CYOKS | 1:19.19 |