| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 3 | MISSION VALLEY HIGH SCHOOL | A | 47.52 | Unverified: |
| 4 | SHAWNEE-MARANATHA ACADEMY | A | 47.04 | Verified |
| 5 | VALLEY FALLS HIGH SCHOOL | A | 47.30 | Unverified: |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | MAUR HILL-MOUNT ACADEMY | A | 46.78 | Verified |
| 3 | EFFINGHAM - ATCHISON COUNTY HIGH | A | DQ | Unverified: Jefferson County North Invitatio |
| 4 | ST. MARY'S HIGH SCHOOL | A | 44.36 | Verified |
| 5 | VALLEY HEIGHTS | A | 45.13 | Verified |
| 6 | JEFFERSON COUNTY NORTH HIGH SCHO | A | 46.55 | Unverified: NEKL |
| 7 | JACKSON HEIGHTS | A | 47.04 | Verified |