| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 2 | BROWN, Marina | MPowered Athletix | NT |
| 3 | CROKER, Zy'Kerriah | Power House Athletics Track & | 28.00 |
| 4 | GAMBLE, Haylee | FLORIDA EAST COAST SUPERSONICS | 25.00 |
| 5 | THOMAS, Seleah | Eastside Xpress | 26.00 |
| 6 | ONUGHA, Nellie | RealDealRunners407 | 28.00 |
| 7 | DOLL, Alexis | RealDealRunners407 | NT |