| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | GIBSON, Brandon | Power House Athletics Track & | 23.00 |
| 2 | TURNER, Amari | Speed Academy | 22.81 |
| 3 | JOHN, Armani | St Pete Nitro Track Club | 22.60 |
| 4 | WILLIAMS, Kevar | Power House Athletics Track & | 22.06 |
| 5 | GREEN JR, Jordan | St Pete Nitro Track Club | 22.56 |
| 6 | LATTIMORE, Andrew | St Pete Nitro Track Club | 22.80 |
| 7 | NEWSOME, Tyrann | St Pete Nitro Track Club | 22.90 |
| 8 | KELLEY, Nolan | E.O.T.O. ELITE | 23.50 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | BASSA, Brycen | Florida Elite/Matrix | 24.00 |
| 2 | HOLMES, Xavier | Space Coast Striders | 23.80 |
| 3 | JOHNSON, David | Empire Athletics | 23.62 |
| 4 | SAINT-FINA, Javon | Power House Athletics Track & | 23.50 |
| 5 | LEWIS, Tyrique | Speed Academy | 23.61 |
| 6 | WESTBROOK, Exavian | Empire Athletics | 23.64 |
| 7 | MCDONALD, Marshae | Speed Academy | 23.94 |
| 8 | LIVINGSTON, Cory | Power House Athletics Track & | 24.50 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | BROWN, Jaylen | Space Coast Striders | 25.20 |
| 2 | LEVARITY, Shalamar | Power House Athletics Track & | 25.00 |
| 3 | RICKERSON, Kenton | Daytona Express Inc | 25.00 |
| 4 | ROBERTS, Nathan | Ivy Hawn Track Club | 24.67 |
| 5 | PUTNAM, Noah | Unattached | 24.80 |
| 6 | WALKER, Jonathan | Unattached | 25.00 |
| 7 | GREAVES, Da'Jon | Fearless | 25.15 |
| 8 | DEOKI, Ray | Speed Academy | 25.60 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | PEREZ, Carlos | Eastside Xpress | NT |
| 2 | PARKER, Walter | Space Coast Striders | NT |
| 3 | RUTH, Lavoris | FLORIDA EAST COAST SUPERSONICS | 28.00 |
| 4 | WILBON, Savion | The Gainesville Striders Track | 26.00 |
| 5 | RYAN, Conner | Unattached | 26.19 |
| 6 | REID, Desmond | Unattached | NT |
| 7 | FIGUEROA III, Manuel | Unattached | NT |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 3 | GOVIA, Jaydyn | Florida Elite/Matrix | NT |
| 4 | JOHNSON-MACK, Gianni | East Orlando Track Club | NT |
| 5 | OXENDINE, Jameel | Daytona Express Inc | NT |