| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | SMALL, Alexis | Speed Academy | 26.03 |
| 2 | WILLIAMS, Demetria | Power House Athletics Track & | 25.80 |
| 3 | HARRIS, Ziona | FLORIDA EAST COAST SUPERSONICS | 25.00 |
| 4 | JOHNSON, Twaneise | V12 Athletics | 24.60 |
| 5 | VREEN, Kalani | Peak Performance Track Club (P | 24.99 |
| 6 | JACKSON, Adina | Fast Lane TC | 25.30 |
| 7 | JULMISTE, Brianna | RealDealRunners407 | 26.00 |
| 8 | ALI, Amira | Unattached | 26.90 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | WILLIAMS, Xaniah | The Gainesville Striders Track | NT |
| 2 | LABORD, Morgan | EVOLUTION SPEED TRACK CLUB | 28.12 |
| 3 | WILLIAMS, Aalaysia | Peak Performance Track Club (P | 27.50 |
| 4 | RIOS, Azaria | QUANTUM TRACK CLUB | 27.00 |
| 5 | SHINGLES, Roslyn | E.O.T.O. ELITE | 27.00 |
| 6 | WATSON-BELL, Allyson | Take Flight Athletics | 28.10 |
| 7 | OXENDINE, Janessa | Daytona Express Inc | 32.60 |