| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | WASHINGTON, Syhion | EVOLUTION SPEED TRACK CLUB | 25.09 |
| 2 | BROWN, Evan | Florida Striders Sprint Club | 24.07 |
| 3 | WARE, Romelo | Train Performance Track Club | 23.50 |
| 4 | WIMBERLY, Jordan | St Pete Nitro Track Club | 22.80 |
| 5 | GIVENS, Nazsir | Blazing Speed Track Club | 23.00 |
| 6 | BURCHETT, Roy | Florida Express Track Inc. | 24.00 |
| 7 | SMITH, Jayshawn | E.O.T.O. ELITE | 25.00 |
| 8 | GORE, Judah | St Pete Nitro Track Club | 25.10 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | PRYOR, Xavian | QUANTUM TRACK CLUB | 26.70 |
| 2 | CLARKE, Ockieve | Dare to Soar TC | 26.18 |
| 3 | JOINER, Ke'Yon | Power House Athletics Track & | 26.00 |
| 4 | COLLANTE, Gabriel | Queen of Peace Catholic Academ | 25.50 |
| 5 | JOHNSON JR, Cedric | Empire Athletics | 25.51 |
| 6 | ROBINSON, Josiah | Blazing Speed Track Club | 26.00 |
| 7 | FLOWERS, Jerius | St Pete Nitro Track Club | 26.70 |
| 8 | DOSTER, Micah | Red Sea Running Club | 28.47 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | TOWNSEND, Jeffrey | Power House Athletics Track & | NT |
| 2 | ROLLE, Alex | Power House Athletics Track & | NT |
| 3 | RUZAKOWSKI, Eddie | Queen of Peace Catholic Academ | NT |
| 4 | BRITTEN, Brennan | Red Sea Running Club | 29.65 |
| 5 | COLE, Kason | Red Sea Running Club | 31.91 |
| 6 | BOYKIN, Travarus | Power House Athletics Track & | NT |
| 7 | GAINES, Sho'Marion | Daytona Express Inc | NT |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 3 | CUNNINGHAM, Jamil | Power House Athletics Track & | NT |
| 4 | SEXTON, Ernest | Manatee Mustangs Track Club | NT |
| 5 | RAY, Caleb | Power House Athletics Track & | NT |