| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | JONES, Cortaryious | Fearless | 27.72 |
| 2 | FREELOVE, Grant | Florida Elite/Matrix | 27.20 |
| 3 | RICHARDSON, Johnny | St Pete Nitro Track Club | 27.00 |
| 4 | SANFORD, Khameron | St Pete Nitro Track Club | 27.00 |
| 5 | SULLIVAN, Armani | St Pete Nitro Track Club | 27.00 |
| 6 | HOLLEY, Issaiah | Power House Athletics Track & | 27.00 |
| 7 | INMON, Ke'Von | St Pete Nitro Track Club | 27.30 |
| 8 | JENKINS, Cunray | St Pete Nitro Track Club | 27.80 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | MORRIS, Joel | Jus' Ryde Track CLub | 31.52 |
| 2 | HEATLY, Steven | NUSPEED II Track Club | 30.00 |
| 3 | KING, Anthony | Red Sea Running Club | 29.67 |
| 4 | GREAVES, Tavion | Fearless | 28.57 |
| 5 | HENDERSON, Christian | Unattached | 29.00 |
| 6 | WRIGHT, Ottoine | RealDealRunners407 | 29.71 |
| 7 | WASHINGTON, Tristen | Central Florida Gliders | 31.27 |
| 8 | VON MAXEY, Alex | Red Sea Running Club | 31.55 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 3 | ROBERTS, Tarryce | Big Lake Track | NT |
| 4 | BARDELL, Jacob | Red Sea Running Club | 34.93 |
| 5 | RICHARDSON, Kaleb | Unattached | 35.71 |
| 6 | ROBERSON, Avery | Space Coast Striders | NT |