| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | FULLER, Makaila | Central Florida Gliders | 34.00 |
| 2 | JONES, Lynnea | St Pete Nitro Track Club | 31.40 |
| 3 | SANGUINE, Angelina | Florida Elite/Matrix | 30.00 |
| 4 | CULPEPPER, Cai | Phase 1 Track Club, Inc. | 28.60 |
| 5 | DENNIS, Kayden | Central Florida Elite | 30.00 |
| 6 | GODFREY, Ny'Omii | W G T Tigers | 31.00 |
| 7 | MCPHERSON, Vahliya | QUANTUM TRACK CLUB | 33.03 |
| 8 | RODRIGUEZ, Jade | East Orlando Track Club | 35.00 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | SUMTER, Hailey | Force One Track | NT |
| 2 | BROOKS, Jasmine | Manatee Mustangs Track Club | NT |
| 3 | HARRIS, Taylor | Dare to Soar TC | NT |
| 4 | LAMPKIN, Chloe | Power House Athletics Track & | 35.00 |
| 5 | WALKER, Sahmyra | QUANTUM TRACK CLUB | 39.44 |
| 6 | DUNCANSON, Alayah | Florida Elite/Matrix | NT |
| 7 | SPLUNGE, Jasmine | East Orlando Track Club | NT |
| 8 | CORBIN, Janiya | Unattached | NT |