| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | MOHR, Prodigy | St Pete Nitro Track Club | 29.75 |
| 2 | SPANN, Ryan | St Pete Nitro Track Club | 29.70 |
| 3 | REESE, Solomon | St Pete Nitro Track Club | 28.40 |
| 4 | RYAN JR, Patrick | Olympia Track Club | 28.00 |
| 5 | SMITH, Ty'Shon | St Pete Nitro Track Club | 28.40 |
| 6 | STALLING, Andre | St Pete Nitro Track Club | 29.70 |
| 7 | SMITH, JR., Kevin | St Pete Nitro Track Club | 29.73 |
| 8 | KINSEY, Tamir | Eastside Xpress | 32.00 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | VALDES-PAGE, Amare | Endurers 4 Christ | 35.00 |
| 2 | WALLACE JR, Phillip | W G T Tigers | 33.15 |
| 3 | FLUELLEN, Dabar | Space Coast Striders | 32.10 |
| 4 | LOWE, Michael | Eastside Xpress | 32.00 |
| 5 | FORD, Eugene | Eastside Xpress | 32.00 |
| 6 | GRAHAM, Cashmere | MPowered Athletix | 32.16 |
| 7 | STEPHENS, Jiaire | Space Coast Striders | 33.50 |
| 8 | ALSTON, Jameirr | Power House Athletics Track & | 36.00 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 2 | FISHER, Mason | Power House Athletics Track & | NT |
| 3 | STARKE, Jayce | Peak Performance Track Club (P | NT |
| 4 | HARRIS, Cameron | NUSPEED II Track Club | 40.00 |
| 5 | FRAZIER, Amir | NUSPEED II Track Club | 41.00 |
| 6 | MARSHALL, Fernando | Big Lake Track | NT |
| 7 | WEAVER, Ayden | E.O.T.O. ELITE | NT |