| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | CLARK, Nina | RealDealRunners407 | NT |
| 2 | ONUGHA, Nellie | RealDealRunners407 | 1:09.00 |
| 3 | THOMAS, Seleah | Eastside Xpress | 1:00.00 |
| 4 | GAMBLE, Haylee | FLORIDA EAST COAST SUPERSONICS | 57.00 |
| 5 | CROKER, Zy'Kerriah | Power House Athletics Track & | 1:00.00 |
| 6 | BROWN, Marina | MPowered Athletix | 1:03.00 |
| 7 | DOLL, Alexis | RealDealRunners407 | NT |