| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | LEVARITY, Shalamar | Power House Athletics Track & | 54.00 |
| 2 | LEWIS, Tyrique | Speed Academy | 52.49 |
| 3 | WILSON, Warren | The Gainesville Striders Track | 51.80 |
| 4 | WILLIAMS, Kevar | Power House Athletics Track & | 49.00 |
| 5 | GOVIA, Jaydyn | Florida Elite/Matrix | 51.00 |
| 6 | MCDONALD, Marshae | Speed Academy | 52.46 |
| 7 | D'ALESSANDRO, Chase | East Orlando Track Club | 53.00 |
| 8 | HOLMES, Xavier | Space Coast Striders | 54.20 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | RAUCCI, Matthew | Red Sea Running Club | 55.88 |
| 2 | GREEN JR, Jordan | St Pete Nitro Track Club | 55.00 |
| 3 | LATTIMORE, Andrew | St Pete Nitro Track Club | 55.00 |
| 4 | LYNN, Remorreyan | St Pete Nitro Track Club | 54.70 |
| 5 | JOHN, Armani | St Pete Nitro Track Club | 54.90 |
| 6 | KELLEY, Nolan | E.O.T.O. ELITE | 55.00 |
| 7 | LANG, Deshawn | Peak Performance Track Club (P | 55.00 |
| 8 | SICO, Aiden | East Orlando Track Club | 57.00 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | RUTH, Lavoris | FLORIDA EAST COAST SUPERSONICS | 1:04.00 |
| 2 | SAINT-FINA, Javon | Power House Athletics Track & | 1:00.00 |
| 3 | CURRY, Larry | Power House Athletics Track & | 1:00.00 |
| 4 | GREAVES, Da'Jon | Fearless | 57.82 |
| 5 | PEREZ, Carlos | Eastside Xpress | 1:00.00 |
| 6 | OXENDINE, Jameel | Daytona Express Inc | 1:00.00 |
| 7 | LARACUENTE, Gabriel | Ivy Hawn Track Club | 1:01.00 |
| 8 | FIGUEROA III, Manuel | Unattached | NT |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 3 | PUTNAM, Noah | Unattached | NT |
| 4 | TURNER, James | East Orlando Track Club | NT |
| 5 | NEWSOME, Tyrann | St Pete Nitro Track Club | NT |