| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | HOLADIA, Carly | East Orlando Track Club | 1:03.00 |
| 2 | HARRIS, Ziona | FLORIDA EAST COAST SUPERSONICS | 1:00.00 |
| 3 | JULMISTE, Brianna | RealDealRunners407 | 59.00 |
| 4 | VREEN, Kalani | Peak Performance Track Club (P | 56.50 |
| 5 | WILLIAMS, Demetria | Power House Athletics Track & | 58.00 |
| 6 | SMALL, Alexis | Speed Academy | 59.15 |
| 7 | ALI, Amira | Unattached | 1:02.00 |
| 8 | LABORD, Morgan | EVOLUTION SPEED TRACK CLUB | 1:04.76 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | GORDON, Madison | EVOLUTION SPEED TRACK CLUB | NT |
| 2 | GREENE, Chloe | Queen of Peace Catholic Academ | 1:18.00 |
| 3 | BOSQUES, Maria | East Orlando Track Club | 1:12.00 |
| 4 | WILLIAMS, Aalaysia | Peak Performance Track Club (P | 1:05.00 |
| 5 | WATSON-BELL, Allyson | Take Flight Athletics | 1:07.00 |
| 6 | OXENDINE, Janessa | Daytona Express Inc | 1:15.00 |
| 7 | WILLIAMS, Xaniah | The Gainesville Striders Track | NT |
| 8 | KLEIN, Savannah | Pure Athletics Ntc | NT |