| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | WASHINGTON, Syhion | EVOLUTION SPEED TRACK CLUB | 1:00.00 |
| 2 | GORE, Judah | St Pete Nitro Track Club | 57.50 |
| 3 | EDGECOMBE, Jaden | E.O.T.O. ELITE | 55.00 |
| 4 | BROWN, Evan | Florida Striders Sprint Club | 54.09 |
| 5 | BURCHETT, Roy | Florida Express Track Inc. | 55.00 |
| 6 | GIVENS, Nazsir | Blazing Speed Track Club | 55.00 |
| 7 | CARTER, Kenneth | Space Coast Striders | 58.50 |
| 8 | TOWNSEND, Jeffrey | Power House Athletics Track & | 1:00.00 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | BARDELL, Justin | Red Sea Running Club | 1:09.23 |
| 2 | ROBINSON, Josiah | Blazing Speed Track Club | 1:04.00 |
| 3 | HINCKLEY, Brady | Red Sea Running Club | 1:02.08 |
| 4 | VANSANT, Landon | Red Sea Running Club | 1:00.52 |
| 5 | WILBON, David | The Gainesville Striders Track | 1:02.00 |
| 6 | CLARKE, Ockieve | Dare to Soar TC | 1:03.02 |
| 7 | BRITTEN, Brennan | Red Sea Running Club | 1:05.76 |
| 8 | STARK, Matthew | Queen of Peace Catholic Academ | 1:10.00 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 2 | ROLLE, Alex | Power House Athletics Track & | NT |
| 3 | GAINES, Sho'Marion | Daytona Express Inc | NT |
| 4 | FULFORD, Khanyea | Power House Athletics Track & | NT |
| 5 | SEXTON, Ernest | Manatee Mustangs Track Club | NT |
| 6 | CARTER, Keyon | Space Coast Striders | NT |
| 7 | WIMBERLY, Jordan | St Pete Nitro Track Club | NT |