| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | WAITE, Andria | Power House Athletics Track & | 1:03.00 |
| 2 | GIBSON, Taylor | Incredibles | 1:02.00 |
| 3 | WILLIAMS, Kehmari | Unattached | 1:00.06 |
| 4 | MCBRIDE, Jaa'Ln | Unattached | 1:00.00 |
| 5 | GAINES, Sanaii | FLORIDA EAST COAST SUPERSONICS | 1:00.00 |
| 6 | MCBRIDE, Jaade | Unattached | 1:01.91 |
| 7 | NOCK, Amirah | Unattached | 1:03.00 |
| 8 | ZEIGLER, Zakilya | NUSPEED II Track Club | 1:04.00 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | GALARZA, Shania | Ivy Hawn Track Club | 1:10.00 |
| 2 | WILLIAMS, Jasmine | Power House Athletics Track & | 1:07.00 |
| 3 | WRIGHT, Adryanna | Peak Performance Track Club (P | 1:06.00 |
| 4 | DORSETT, Samiah | NUSPEED II Track Club | 1:06.00 |
| 5 | CURRY, Laura | Power House Athletics Track & | 1:06.00 |
| 6 | JOHNSON, Ruth | EVOLUTION SPEED TRACK CLUB | 1:06.01 |
| 7 | KING, Madalyn | Central Florida Gliders | 1:09.00 |
| 8 | JENKINS, Akeira | St Pete Nitro Track Club | 1:10.00 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | ESPADA, Sariah | Power House Athletics Track & | 1:15.00 |
| 2 | BRIONEZ, Hailey | Red Sea Running Club | 1:14.48 |
| 3 | NEAL, Nierica | FLORIDA EAST COAST SUPERSONICS | 1:13.00 |
| 4 | TAYLOR, Keionna | Endurers 4 Christ | 1:12.00 |
| 5 | LOZANO, Galilea | Red Sea Running Club | 1:12.28 |
| 6 | KEMP, Corinne | Red Sea Running Club | 1:13.37 |
| 7 | SMITH, Ivory | St Pete Nitro Track Club | 1:15.00 |
| 8 | COMBS, Callie | Red Sea Running Club | 1:20.79 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 2 | WRIGHT, Taniyah | Manatee Mustangs Track Club | NT |
| 3 | WILLIAMS, Ayana | MPowered Athletix | NT |
| 4 | GREEN, Leneah | Fearless | NT |
| 5 | PIERRE, Isis | Power House Athletics Track & | NT |
| 6 | WILLIAMS, Alivia | Empire Athletics | NT |
| 7 | FRAZIER, Jordyn | FITFAM Elite | NT |