| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | HENRIQUES, Rajkumar | RealDealRunners407 | 1:05.00 |
| 2 | CURRY, Lawrence | Power House Athletics Track & | 1:00.00 |
| 3 | MURRAY, Lamichael | Power House Athletics Track & | 1:00.00 |
| 4 | SANFORD, Khameron | St Pete Nitro Track Club | 59.00 |
| 5 | SULLIVAN, Armani | St Pete Nitro Track Club | 1:00.00 |
| 6 | RICHARDSON, Johnny | St Pete Nitro Track Club | 1:00.00 |
| 7 | HENDERSON, Christian | Unattached | 1:00.06 |
| 8 | WRIGHT, Ottoine | RealDealRunners407 | 1:06.00 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | JONES, Damarion | Central Florida Elite | 1:23.00 |
| 2 | HEATLY, Steven | NUSPEED II Track Club | 1:14.00 |
| 3 | WASHINGTON, Tristen | Central Florida Gliders | 1:09.94 |
| 4 | ROBERSON, Avery | Space Coast Striders | 1:07.00 |
| 5 | MINNIS, Mikhael | Daytona Express Inc | 1:07.00 |
| 6 | HARRIS, Cecil | FLORIDA EAST COAST SUPERSONICS | 1:10.00 |
| 7 | JENKINS, Cunray | St Pete Nitro Track Club | 1:15.00 |
| 8 | SEXTON, Christian | Manatee Mustangs Track Club | NT |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 3 | HAGER, Lamor | W G T Tigers | NT |
| 4 | SPLUNGE, Jaden | East Orlando Track Club | NT |
| 5 | GREAVES, Tavion | Fearless | NT |