| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Crews Lake Middle School | A | Finch, John | Harrison, Omarion | Rosheed, Ahmed | Starks, LaDamien | NT |
| 3 | Paul R. Smith Middle School | A | Goss, Gage | Rivera, Steven | Bigler, Sonny | Robinson, Jace | DQ |
| 4 | Gulf Middle School | A | Adams, Keonte | Wynn, Dontavius | Blanton, AJ | Jones, Austin | 55.44 |