| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 4 | West Port |
B | Guzman | Andall | Davis JR | Marshall-Cain | NT |
| 5 | Buchholz |
B | Johnson | Etienne | Hickey | Shaw | 43.82 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Wildwood |
A | Slowley | Keiler | Patterson | Turner | NT |
| 2 | West Port |
A | Coleman | Honor | Henderson | Henry | 44.50 |
| 3 | Buchholz |
A | Carter | White | Trapp | hicks | 43.82 |
| 4 | Vanguard (Ocala) |
A | Bernard | Brown | Gary | Gaskin | 43.37 |
| 5 | Lake Minneola |
A | Parker | Saint-Felix | Rodgers | Patel | 43.65 |
| 6 | Seven Rivers Christian |
A | Graham | Howard | Kuntz | Cepeda | NT |
| 7 | Meadowbrook |
A | Daughtery | Ross | Parchment | Anson | NT |
| 8 | Leesburg |
A | Thompson | Varner | Campbell | Villango | NT |